A Case Control Study to Evaluate the Sensitivity of Salivary over Serum Glycated Protien Levels in Patients with Controlled Type 2 Diabetes Mellitus
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Abstract
Background: To evaluate the sensitivity of salivary over serum glycated protein levels in patients with type 2 diabetics mellitus.
Materials and methods: The samples for the present study comprised of two groups. Group A included 60 Age and sex matched healthy adult whereas group B consisted of 60 adults with controlled type2 diabetes mellitus. Blood samples (1.5 ml of venous blood) are collected with EDTA in the test tubes for the determination of glycosylated hemoglobin and Serum glucose level. Stimulated saliva was collected from both groups in a sterile container. Then the salivary samples were also subjected to glucose estimation and glycosylated hemoglobin by microcolumn method. Serum fructosamine and salivary fructoseamine was estimated by NBT reduction method (B.L. Somani et al 2010) using Schimadzu CL-750 Spectrophotometer and Colorimeter.
The results were statistically evaluated with one way ANOVA, Pearson’s correlation test.
Results: The salivary fructoseamine levels were significantly increased in the case groups, when compared with controls. There was an increase in the salivary fractoseamine levels with an increase in HbA1c percentage. The correlation analysis revealed a significant positive correlation(P < 0.001) between salivary and serum glucose levels and salivary glucose level with HbA1c.
Conclusion:Diabetes is known to influence salivary composition and function and therefore salivary fructoseamine can be used as a noninvasive diagnostic aid for diagnosis and monitoring of diabetes.