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Aim: The research study was done for the evaluation of the time taken for decalcification of four decalcifying solutions and their worsening effect on staining characteristics.
Materials and Methods: four different decalcifying solutions namely, 37 % HCl, 5% Nitric acid,5 % Formic acid, 14% Neutral Ethylene Diamine Tetra Acetic acid (EDTA)were used for decalcification of 30 freshly extracted teeth. Decalcification end point was assessed by means of chemical method. Then teeth were processed routinely and stained H & E stain. Results: Neutral EDTA was the most innocent to the tissues architectural preservation and 37 % HCL was the least one. Conclusions: Neutral EDTA, in spite of being the slowest solution among all, gave very good results with respect to structural integrity, and excellent quality of staining.
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