Comparison of Different Decalcifying Solutions on Human Teeth

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Published: May 29, 2023
Keywords:
Teeth decalcification HCL formic acid nitric acid neutral EDTA decalcifying solution pulp integrity

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Nileshwariba Jadeja
PhD scholar, Department of Oral Pathology and Microbiology, Gujarat University, Ahmedabad, Gujarat, India.
Neelampari Parikh
Associate professor, Department of Oral Pathology and Microbiology, DDU Dental college, Nadiad, Gujarat, India.
Stuti Bajaj
Senior lecturer, Department of Oral Pathology and Microbiology,Karnavati school of dentistry,Karnavati university, Gandhinagar, Gujarat, India.
Hemant Kale
Private practitioner, Ahmedabad, Gujarat, India.
Anil Patel
Reader, Department of Oral Pathology and Microbiology, Karnavati school of dentistry, Karnavati university, Gandhinagar, Gujarat, India.

Abstract

Introduction: In histopathology practice, decalcification is an important procedure during  processing of a specimen. Number of decalcifying solutions were used till now but the potential and effect of it on the tissue and speed of decalcification are the basic pillars for the selection of a decalcifying solution. Some of the solutions eliminate the calcium rapidly and efficiently but they are detrimental for staining characteristics.
Aim: The research study was done for the evaluation of the time taken for decalcification of four  decalcifying solutions and their worsening effect on staining characteristics.

Materials and Methods: four different decalcifying solutions namely, 37 % HCl, 5% Nitric acid,5 % Formic acid, 14% Neutral Ethylene Diamine Tetra Acetic acid (EDTA)were used for decalcification of 30 freshly extracted teeth. Decalcification end point was assessed by means of chemical method. Then teeth were processed routinely and stained H & E stain. Results: Neutral EDTA was the most innocent to the tissues architectural preservation and 37 % HCL was the least one. Conclusions: Neutral EDTA, in spite of being the slowest solution among all, gave very good results with respect to structural integrity, and excellent quality of staining.

Article Details

How to Cite
Nileshwariba Jadeja, Neelampari Parikh, Stuti Bajaj, Hemant Kale, & Anil Patel. (2023). Comparison of Different Decalcifying Solutions on Human Teeth. Journal of Coastal Life Medicine, 11(2), 532–539. Retrieved from https://www.jclmm.com/index.php/journal/article/view/1044
Issue
Vol. 11: Number 2, 2023
Section
Articles

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