Comparison of Different Decalcifying Solutions on Human Teeth

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Nileshwariba Jadeja
Neelampari Parikh
Stuti Bajaj
Hemant Kale
Anil Patel


Introduction: In histopathology practice, decalcification is an important procedure during  processing of a specimen. Number of decalcifying solutions were used till now but the potential and effect of it on the tissue and speed of decalcification are the basic pillars for the selection of a decalcifying solution. Some of the solutions eliminate the calcium rapidly and efficiently but they are detrimental for staining characteristics.
Aim: The research study was done for the evaluation of the time taken for decalcification of four  decalcifying solutions and their worsening effect on staining characteristics.

Materials and Methods: four different decalcifying solutions namely, 37 % HCl, 5% Nitric acid,5 % Formic acid, 14% Neutral Ethylene Diamine Tetra Acetic acid (EDTA)were used for decalcification of 30 freshly extracted teeth. Decalcification end point was assessed by means of chemical method. Then teeth were processed routinely and stained H & E stain. Results: Neutral EDTA was the most innocent to the tissues architectural preservation and 37 % HCL was the least one. Conclusions: Neutral EDTA, in spite of being the slowest solution among all, gave very good results with respect to structural integrity, and excellent quality of staining.

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Nileshwariba Jadeja, Neelampari Parikh, Stuti Bajaj, Hemant Kale, & Anil Patel. (2023). Comparison of Different Decalcifying Solutions on Human Teeth. Journal of Coastal Life Medicine, 11(2), 532–539. Retrieved from


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