Development and Validation of HPLC Method of Gabapentin

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Published: May 29, 2023
Keywords:
HPLC UV Spectroscopy Gabapentin Development Validation GBP

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Manjusha Aher
Amrutvahini College of Pharmacy, Sangamner, Amrutnagar, Sangamner, Ahmednagar, Maharashtra, 422608, India
Madhuri Balasaheb Kanawade
Amrutvahini College of Pharmacy, Sangamner, Amrutnagar, Sangamner, Ahmednagar, Maharashtra, 422608, India
Sarika Bhabad
Samarth College of Pharmacy, Belhe, Nagar-Kalyan Highway, Pune, Maharashtra, 422606, India
Ramanlal N. Kachave
GES’s Sir Dr. M. S. Gosavi College of Pharmaceutical Education and Research Nashik, Affiliated Savitribai Phule Pune University, Pune, Nashik, Maharashtra, 422005, India

Abstract

Gabapentin, an anticonvulsant, is commonly used to treat epilepsy. Gabapentin lacks a chromophore, making its absorption very low and complicating its analysis and reducing its sensitivity. Chemical derivatization adds a chromophore, allowing HPLC to identify and quantify the medication at much lower concentrations.  An auxochrome group added a chromophore to gabapentin to derivatize it. Gabapentin was catecholized using coupling reagents. HPLC with UV/Vis detector developed the analytical method. Linearity, range, precision and accuracy were also validated.  Gabapentin was derivatized using catechol reagent at 300 nm. HPLC employed 50:50 methanol-water mobile phase. The gabapentin eluted peak was isolated from other derivatization reagents. The analytical method was validated and met all validation requirements. The approach was linear (R2 = 0.9994), precise (RSD = 1.54), and accurate (99-103% recovery). It's straightforward and sensitive. It can analyse gabapentin in various dose forms and APIs. This research can be continued and utilised to evaluate gabapentin in biological systems.

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How to Cite
Manjusha Aher, Madhuri Balasaheb Kanawade, Sarika Bhabad, & Ramanlal N. Kachave. (2023). Development and Validation of HPLC Method of Gabapentin . Journal of Coastal Life Medicine, 11(2), 632–643. Retrieved from https://www.jclmm.com/index.php/journal/article/view/1059
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Vol. 11: Number 2, 2023
Section
Articles

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