Analysis and Determination of the Proliferative Biomarker Potential of Ki-67 in Cervical Lession/Cervical Cancer using Immunohistochemical Method.

Main Article Content

Christy Chinyere Fredrick
Obum-Nnadi Charity Ndidi
Kelechukwu Sylvester Nwokorie
Dennis Amaechi
Jonathan U. Madukwe
Nnawuihe Chika Winnifred
Ravi Kanna
Rajeeve Kumar
Anurdha Talukdar
Litika Vermani
Poulome Mukherjee
Humphery B. Osadulor


Background: Cervical cancer is believed to be one of the most widespread cancers worldwide with the utmost burden prevalent among women in areas where resources are scarce.

Methods:The study population was from 10 years  retrospective archival tissue blocks of cervical cancer among women  in Abuja metropolis from 2005 to 2015 Histopathological diagnosis by H and E was made, from  the 80 cases examined 20(25%) Cases  were cervicitis (CC), 15(18.8%) were cervical intraepithelial neoplasia (CINI), 15(18.8%) were cervical intraepithelial neoplasia (CIN2) while 30(37.3%) cases were squamous cell carcinoma SCC They were examined immunohistochemically by Avidin Biotin Complex (ABC) method. Ki67 (proliferation marker)

Results: A statistically significant rate of positivity was found in the expression of Ki-67 among all the categories of the studied cases.

Conclusion: This study showed that most of the immunological markers examined can be used for identification of different stages involved in cancer progression, also for diagnosis of cancer, predictive prognosis and can be explore as therapy targets for cervicitis and Squamous cell carcinoma.

Article Details

How to Cite
Christy Chinyere Fredrick, Obum-Nnadi Charity Ndidi, Kelechukwu Sylvester Nwokorie, Dennis Amaechi, Jonathan U. Madukwe, Nnawuihe Chika Winnifred, Ravi Kanna, Rajeeve Kumar, Anurdha Talukdar, Litika Vermani, Poulome Mukherjee, & Humphery B. Osadulor. (2023). Analysis and Determination of the Proliferative Biomarker Potential of Ki-67 in Cervical Lession/Cervical Cancer using Immunohistochemical Method. Journal of Coastal Life Medicine, 11(2), 1371–1381. Retrieved from


Spradling , A.C & Sun, J. (2013). Ovulation in Drosophila is controlled by secretory cells of the female reproductive tract. Elife, 2, e00415.

Bray, Freddie. (2013). "Worldwide Trends in Cervical Cancer Incidence: Impact of Screening against Changes in Disease Risk Factors". European Journal of Cancer. 49 (15): 3262–73.

Jain MA, Limaiem F. Cervical Squamous Cell Carcinoma. [Updated 2023 Jan 1]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 Jan-. Available from:

Mukama, T., Ndejjo, R., Musabyimana, A., Halage, A. A.&Musoke, D. (2017). Women’s knowledge and attitudes towards cervical cancer prevention: a cross sectional study in Eastern Uganda. BMC women's health.17(1), 9.

Sobecki M, Mrouj K, Camasses A, Parisis N, Nicolas E, Llères D, Gerbe F, Prieto S, Krasinska L, David A, Eguren M, Birling MC, Urbach S, Hem S, Déjardin J, Malumbres M, Jay P, Dulic V, Lafontaine DLj, Feil R, Fisher D (2016). The cell proliferation antigen Ki-67 organises heterochromatin. Elife,5:e13722. doi: 10.7554/eLife.13722. PMID: 26949251; PMCID: PMC4841783.

Aman NA, Doukoure B, Koffi KD, Koui BS, Traore ZC, Kouyate M, Toure I, Effi AB. Immunohistochemical Evaluation of Ki-67 and Comparison with Clinicopathologic Factors in Breast Carcinomas. Asian Pac J Cancer Prev. 2019 Jan 25;20(1):73-79. doi: 10.31557/APJCP.2019.20.1.73. PMID: 30678383; PMCID: PMC6485584.

Takkem A, Barakat C, Zakaraia S, Zaid K, Najmeh J, Ayoub M, Seirawan MY. Ki-67 Prognostic Value in Different Histological Grades of Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma. Asian Pac J Cancer Prev. 2018 Nov 29;19(11):3279-3286. doi: 10.31557/APJCP.2018.19.11.3279. PMID: 30486632; PMCID: PMC6318382.

Aslani, F. S., Safaei, A., Pourjabali, M., &Momtahan, M. (2013). Evaluation of Ki67, p16 and lesions. Iranian Journal of Medical Sciences, 38:(1) 15.

Pan D, Wei K, Ling Y, Su S, Zhu M, Chen G. (2015). The prognostic role of Ki-67/MIB-1 in cervical cancer: a systematic review with meta-analysis. Med Sci Monit. 21:882-9. doi: 10.12659/MSM.892807. PMID: 25807305; PMCID: PMC4386420.

Krtinic, D., Zivadinovic R., Jovic, Z., Pesic, S., Mihailovic, D., Ristic I., Cvetanovic, A., Todorovska, I., Zivkovic N., Rankovic, G. N., Stokanovic D., Zivadinovic B., Trandafilovic M., Apostolovic M. A., Golubovic, M., Zivadinovic, A. (2018). Significance of the Ki-67 proliferation index in the assessment of the therapeutic response to cisplatin-based chemotherapy in patients with advanced cervical cancer. European Review for Medical and Pharmacological Sciences, 22: 5149-5155

Chen M, Yao S, Cao Q, Xia M, Liu J, He M. (2016). The prognostic value of Ki67 in ovarian high-grade serous carcinoma: an 11-year cohort study of Chinese patients. Oncotarget., 8(64):107877-107885. doi: 10.18632/oncotarget.14112. PMID: 29296209; PMCID: PMC5746111.

Ayad Ahmad Mohammed (2019). Quantitative assessment of Ki67 expression in correlation with various breast cancer characteristics and survival rate; cross sectional study. Annals of Medicine and Surgery,48: 129-134.

Han-Yu Deng, Zi-Hang Chen,Zhi-Qiang Wang, Yun-Cang Wang, En-Min Li, Li-Yan Xu, Yi-Dan Lin, and Long-Qi Chen (2017). High expression of Ki-67 is an independent favorable prognostic factor for esophageal small cell carcinoma. Oncotarget. 8(33): 55298–55307doi: 10.18632/oncotarget.19426.

Wang D, Chen D, Zhang C, Chai M, Guan M, Wang Z, Wei Y, Zhang Y, Shi Q, Gu K. (2020). Analysis of the relationship between Ki-67 expression and chemotherapy and prognosis in advanced non-small cell lung cancer. Transl Cancer Res.,9(5):3491-3498. doi: 10.21037/tcr.2020.03.72. PMID: 35117714; PMCID: PMC8798892.

Si, M., Lang, J. The roles of metallothioneins in carcinogenesis. J Hematol Oncol 11, 107 (2018).

Hosseini MS, Talayeh M, Afshar Moghaddam N, Arab M, Farzaneh F, Ashrafganjoei T. (2023). Comparison of Ki67 index and P16 expression in different grades of cervical squamous intraepithelial lesions. Caspian J Intern Med.Winter,14(1):69-75. doi: 10.22088/cjim.14.1.69. PMID: 36741489; PMCID: PMC9878899.