Synthesis of Herbal Solution Based on Thymus Kotschyanus as an Antimicrobial Agent Against Enterococcus Faecalis and Streptococcus Mutans Isolated from the Periapical Cases and Comparison of its Antimicrobial Effect with Antibiotics and Chlorhexidine

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Helia Shahbazboroojerdi
Mahbod Shahabedin
Ashkan Salehi


Background: Thymus kotschyanus is an natural essential oil with the boost antimicrobial effects. The present research was aimed to assess the antimicrobial effects of T. kotschyanus essential oil, chlorhexidine 0.2% mouthwash and antibiotic agents against Enterococcus faecalis and Streptococcus mutans isolated from the periapical cases in vitro condition.
Methods: Aerial parts of the T. kotschyanus were prepared and used for essential oil extraction. Antimicrobial effects were examined using the disk diffusion and MIC and MBC determination.
Results: The diameter of the growth inhibition zones had ranges between 4.17±0.19 to 11.90±0.24 mm. The highest diameter of the growth inhibition zone was recorded for E. faecalis treated with T. kotschyanus (10 mg/ml), while the lowest was found for S. mutans treated with T. kotschyanus (2.5 mg/ml). Statistically significant differences were not obtained between some concentration of the T. kotschyanus and chlorhexidine, ampicillin, amoxicillin, and metronidazole antibiotic agents (P >0.05). The MIC values had ranges between 2.5 to 5 mg/ml. The MBC values ranges between 5 to 10 mg/ml. The lowest MIC was identified for E. faecalis treated by T. kotschyanus essentiall oil (2.5 mg/ml).

Conclusion: Overall, in our study it was shown that the essential oil of thyme T. kotschyanus at a concentration of 10mg/ml.It has effective antimicrobial effects on clinical isolates of S. mutans and E. faecalis. However, the antimicrobial effects of T. kotschyanus essential oil were similar to chlorhexidine 0.2% and antibiotics such as ampicillin, amoxicillin, and metronidazole. But note It is important that the essential oil is natural and does not have any chemicals or side effects, which can be recommended as a mouthwash, especially for periapical infection patients

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Helia Shahbazboroojerdi, Mahbod Shahabedin, & Ashkan Salehi. (2023). Synthesis of Herbal Solution Based on Thymus Kotschyanus as an Antimicrobial Agent Against Enterococcus Faecalis and Streptococcus Mutans Isolated from the Periapical Cases and Comparison of its Antimicrobial Effect with Antibiotics and Chlorhexidine. Journal of Coastal Life Medicine, 11(2), 1643–1651. Retrieved from


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