Effect of Incorporation of Clotrimazole on the Zone of Inhibition of Candida Albicans and Surface Roughness in Permanent Soft Liners

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Kalpesh Vaishnav
Dhaara Rana
Ravi Joshi
Radhika Agnihotri
Dipti Shah
Riya Patel

Abstract

Purpose: Permanent soft liners used for the treatment of denture stomatitis have the drawback of increased porosity and surface roughness. This creates a niche for the growth of opportunistic infections. This study aimed to analyze the change in the zone of inhibition and surface roughness with the addition of clotrimazole in soft liner.  Materials and methods :Long-term permanent soft liners Permasoft (group 1 and 3) and Mollosil (group 2 and 4) were used to fabricate specimen discs (n=48/group) and blocks(n=8/group) with and without clotrimazole in an aseptic environment using a die. Samples were stored in salivary substitute. Discs were placed in petri dishes containing candida albicans culture on sabouraud dextrose agar medium and incubated for 24 hrs. to measure the zone of inhibition. Surface roughness tester -SJ-210 Mitutoyo Instrument was used on blocks to measure surface roughness. Readings were recorded at time intervals of 1, 7,14,21,35,42 days.Results:One way ANOVA test, post hoc Tukey analysis, and Student t-test were performed. After 42 days decrease in the zone of inhibition was observed (p<0.001) for group 2 and 4 and a constant increase in surface roughness for all groups (p<0.001) Zone of inhibition in control groups was nil. Conclusion:The addition of clotrimazole in soft liner inhibits the growth of candida albicans in vitro and can be used for treating candidiasis. Surface roughness values increase within the clinically acceptable limits.

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How to Cite
Kalpesh Vaishnav, Dhaara Rana, Ravi Joshi, Radhika Agnihotri, Dipti Shah, & Riya Patel. (2022). Effect of Incorporation of Clotrimazole on the Zone of Inhibition of Candida Albicans and Surface Roughness in Permanent Soft Liners. Journal of Coastal Life Medicine, 10(3), 250–257. Retrieved from https://www.jclmm.com/index.php/journal/article/view/187
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References

Karin hermana Neppelenbroek,Jozely Francisca MelloLim, JulianaHotta,Lucas Lulo Galitesi, Ana Lucia Pompeia Fraga Almeida, Vanessa Migliorini Urban. effect of incorporation of antifungal agents on the ultimate tensile strength of temporary soft denture liners. Journal of Prosthodontics 2017;27(2):177-81.

Naval et. al. Complete denture construction based. JPD 1958;9(4); 539-551.

Rodrigues s, Shenoy et. al. Resilient liners : a review. JIPS 2013;13(123):155–64.

Bhatt, M., Shah, D., Vaishnav, K., Harsolia, Z., & Dangi, R. (2022). An in-vitro study of the effect of incorporation of antifungal drugs upon the growth of candida and surface hardness in permanent soft liners. International Journal of Health Sciences, 6(S2), 3141–3155. https://doi.org/10.53730/ijhs.v6nS2.5761

Bueno MG, Juliana , Sousa, Hotta. Surface properties of temporary soft liners modified by minimum inhibitory concentrations of antifungals. Oral Diseases. 2017;28:158–64.

Datta K, Devi et. al. Comparative evaluation of the effect of clotrimazole and fluconazole incorporated into two auto-polymerizing acrylic-based denture liners on the growth and colonization of candida albicans specimen fabrication. International Journal of Innovation Sciences and Research 2016;5:810–5.

Schneid TR. Student scientific award paper. 1992;12(6).

Neppelenbroek KH. Effect of incorporating antifungals on the water sorption and solubility of interim resilient liners for denture base relining. J prosthetic dent. 2013;1–6.

Urban VM, Thiago F, Bueno. Effect of the addition of antimicrobial agents on shore hardness and roughness of soft lining materials. Oral Diseases 20145,21;57-65

Alcântara , Macêdo , Gurgel, Jorge, Karin , Urban. Peel bond strength of resilient liner modified by the addition of antimicrobial agents to denture base acrylic resin. J applied Oral Science 2012;20(6):607–12.

Gregory Parr et. al. In vitro hardness, water sorption, and resin solubility of laboratory-processed and auto polymerized long-term resilient denture liners over one year of water storage.JPD 2002,139–44.

Grzegorz et al. Long-term soft denture lining materials. Materials 2014;5816–42.

Street T., Wright et. al. A three-year longitudinal study of denture soft lining materials in clinical use. Clinical Materials 1986;281–9.

Bueno mg, Urban VM, Barb gs, Silva WJ, Porto VC, Pinto l, et al. Effect of antimicrobial agents incorporated into resilient denture relines on the candida albicans biofilm. Oral Diseases 2015;57–65.

Pachavaet. al. Comparative antifungal efficacy of denture soft liners with clotrimazole : an invitro study abstract : Indian Journal Of Dental Advancement 2014; 1593-1595.

Sholapurkar aa, pai km, rao s. Comparison of efficacy of fluconazole mouth rinse and clotrimazole mouth paint in the treatment of oral candidiasis. Australian Dental Journal 2009; August:341–6.

Abbas Falah et al.A comparison of the efficacy of nystatin and fluconazole incorporated into tissue conditioner on the in vitro attachment and colonization of candida albicans. Dent Res J 2010; 7(1): 18-22

Abbas Falah et al. A comparison of the efficacy of nystatin and fluconazole incorporated into tissue conditioner on the in vitro attachment and colonization of. Dent Res J 2010; 7(1): 18-22.

Braden M et. al. Tissue conditioners : Composition and structure. Adv Dental Research 1988;2(l):93-97.

Braden M. et. al. Some aspects of the chemistry and physics of dental resins.Adv Dental Research 1988;93–7.

P S Wright. Composition and properties of soft lining materials for acrylic dentures J. of Dentistry. 1981;(3):210–23.

M Addy et. al. In vitro studies into the use of denture base and soft liner materials as carriers for drugs in the mouth.J. Of OralRehabilitation 1981;8:131–42.

Radnai M, Whiley R, Friel T, Wright PS. Effect of antifungal gels incorporated into a tissue conditioning material on the growth of candida albicans. J. of Dentistry 2010;292–6.

Vojdani M, Zibaei m, Zomorodian K. In- vitro study of the effect of clotrimazole incorporation into silicone soft liner on fungal colonization.Shiraz Uni Dent Journal 2009;9:19–23.

Machado, Breeding, Vergani, Elias. Hardness and surface roughness of relining and denture base acrylic resins after repeated disinfection procedures. J prosthetic dent 102(2):115–22.

Johnson, Glenn, et. al. Effects of chemical disinfectants on the surface characteristics and color of denture resins. J Prosthet Dent 1997;77:197-204.

Habib , Machado al, Giampaolo et,Vergani. Surface roughness of denture base and reline materials after disinfection by immersion in chlorhexidine or microwave irradiation. The Gerodontology Society and John Wiley & Sons A/S, Gerodontology 2012; 2012;375–8