Controlled Release Studies of Immobilized Lysozyme from Polyelectrolyte Multilayer Membrane Sunder Different PH Conditions
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Abstract
In the present study, potential application of Chitosan/Polystyrene sulfonate multilayermembrane as reservoirs for releasinga protein in a controlled manner is explored by carrying out the desorption study, especially the time dependent desorption study of lysozyme immobilised on the multilayer at two selected pH 8.8 and 10.6. The desorption studies indicates that the protein is reversibly bound to the polyelectrolyte multilayer for the studied pH, 8.8 and 10.6. It was observed that the desorption of protein increases progressively as the ionic strengthincreases and almost complete desorption occurred when the salt concentration reaches 0.5 M NaCl. The data obtained from UV and FT-IR studies shows that there is difference in the desorption pattern for the lysozyme adsorbed membranes under the two pH conditions. As lysozyme finds medicinal applications, the present work reveals that controlled drug delivery systems can be fabricated using this polyelectrolyte multilayer system.
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