Evaluation of Antimicrobial Efficacy of Andrographis Paniculate (Nilavembu Decoction) against Various Microbial Biofilm Formed on the Tooth Substrate -An in Vitro Study
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Aim: To evaluate the antimicrobial efficacy of Andrographis paniculata (nilavembu decoction) against various microbial biofilm formed on tooth substrate and to compare it with gold standard chlorhexidine mouthwash
Design: An in vitro experimental study
Methods and Material: An experimental invitro study was conducted among six test pathogens that include 3-gram positive bacteria, 2-gram negative bacteria and fungi. The anti-microbialproperty was assessed by the Zone of Inhibition, Minimum Inhibitory Concentration and the number of colonies formed on the tooth substrate on 3rd day and 5th day was assessed as Colony Forming Units per milliliter (CFU/ml) and comparison between the nilavembu decoction, chlorhexidine and saline was done.
Statistical analysis used: All the microbiological procedures were repeated thrice and the mean and standard deviation was calculated. Kruskall Wallis ANOVA was used for comparison of number of colonies forming units of various microorganisms between three groups followed by Mann Whitney U test as post hoc test for pair wise comparison
Results: The results showed that nilavembu decoction has showed larger zone of inhibition(16.1mm) compared to chlorhexidine (14.3mm) and it was statistically significant (P<0.05) Nilavembu decoction also showed its effect at lesser concentration against Candida albicans (50mg/ml) and Enterococcus faecalis (25mg/ml) and reduced number of microbial colonies was found on 3rd day microbial biofilm compared to 5th day biofilm which shows that nilavembu decoction have better effect on immature biofilm.
Conclusions:Nilavembu decoction showed better antimicrobial effect against Candida albicans, Enterococcus faecalis and Streptococcus mutans compared to other test pathogens.
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