Preparation of Crude Extract from Chaetomorpha Species Seaweed and Analysis of their Antioxidant Property - An in Vitro Study
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INTRODUCTION: Chronic periodontitis is a leading cause of tooth loss leading to poor quality of life worldwide. Dental plaque biofilm is the primary etiologic factor for the development of periodontal infections. The ensuing interaction between the plaque microflora and the host results in cascades of events ultimately leading to an inflammatory response in the periodontal tissues. Antioxidants are molecules that fight free radicals in our body. Chaetomorpha species, a common and widespread green seaweed genus with unbranched filaments. Its wide variety of bioactive substances makes it a potential candidate to explore its antioxidant properties. AIM: To analyze the antioxidant property of crude extract prepared from chaetomorpha species MATERIALS AND METHODS: The study was conducted at the Department of Biomaterials, Saveetha Dental College. Cheatomorpha species seaweed was collected from the Bay of Bengal ocean along the Chennai coastline and ethanolic crude extract was prepared. The extract was then analyzed for antioxidant activity using DPPH assay, H2O2 radical scavenging test and total antioxidant analysis. Control used was ascorbic acid. RESULTS: Antioxidant activity gradually increased in accordance to the increase in concentration of the sample. The highest concentration of 100ug/ml showed 64% of antioxidant activity whereas ascorbic acid which is taken as control showed 80% of antioxidant activity at 100ug/ml concentration. CONCLUSION: Natural resources are often used for discovery of drugs and play a vital role in programs of drug development. The biological activities of marine algae are increasing tremendously. Therefore, mechanical therapy alone is not sufficient in the treatment of periodontal infections. In severe periodontitis, adjunct therapy in the form of antioxidant supplements would help to overcome the tissue destruction mediated oxidative stress and bring the tissues back to homeostasis.
Dahiya P, Bhardwaj R, Chaudhary K, Kamal R, Gupta R, Kaur S. Reactive oxygen species in periodontitis [Internet]. Vol. 17, Journal of Indian Society of Periodontology. 2013. p. 411. Available from: http://dx.doi.org/10.4103/0972-124x.118306
Periodontitis [Internet]. 2020. Available from: http://dx.doi.org/10.3390/books978-3-03943-508-1
Halliwell B. Reactive oxygen species in living systems: Source, biochemistry, and role in human disease [Internet]. Vol. 91, The American Journal of Medicine. 1991. p. S14–22. Available from: http://dx.doi.org/10.1016/0002-9343(91)90279-7
Canakci CF, Cicek Y, Canakci V. Reactive Oxygen Species and Human Inflammatory Periodontal Diseases [Internet]. Vol. 70, Biochemistry (Moscow). 2005. p. 619–28. Available from: http://dx.doi.org/10.1007/s10541-005-0161-9
Hafeez N. Assessment of Oxidative Stress and Antioxidant Levels in Chronic Periodontitis Patients [Internet]. Vol. 35, Alinteri Journal of Agricultural Sciences. 2020. p. 151–5. Available from: http://dx.doi.org/10.47059/alinteri/v35i2/ajas20087
Wang Y, Andrukhov O, Rausch-Fan X. Oxidative Stress and Antioxidant System in Periodontitis [Internet]. Vol. 8, Frontiers in Physiology. 2017. Available from: http://dx.doi.org/10.3389/fphys.2017.00910
Gomathy J, Jayalakshmi L, Jayanthi J, Ragunathan MG. An in Vitro Study on The Antimicrobial Activity and Antioxidant Activities of The Extract of A Seaweed, Enteromorpha Intestinalis Against Certain Pathogens [Internet]. Available from: http://dx.doi.org/10.21203/rs.3.rs-1116593/v1
Kanzaki H, Wada S, Narimiya T, Yamaguchi Y, Katsumata Y, Itohiya K, et al. Pathways that Regulate ROS Scavenging Enzymes, and Their Role in Defense Against Tissue Destruction in Periodontitis [Internet]. Vol. 8, Frontiers in Physiology. 2017. Available from: http://dx.doi.org/10.3389/fphys.2017.00351
Research CM, Case Medical Research. Anti Oxidant Enzymes and Total Antioxidant Levels in Diabetes Mellitus Type II Patients and Healthy Individuals With Periodontitis [Internet]. Case Medical Research. 2019. Available from: http://dx.doi.org/10.31525/ct1-nct04180332
Kallswari G, Mahendran S, Subalakshmi P, Shankar T, Ponmanickam P. Purification, Characterization and Antioxidant Activity of Green Seaweed Codium sp [Internet]. Vol. 4, Advances in Pharmacology and Pharmacy. 2016. p. 16–21. Available from: http://dx.doi.org/10.13189/app.2016.040202
Athiperumalsami T, Devi Rajeswari V, Hastha Poorna S, Kumar V, Louis Jesudass L. Antioxidant activity of seagrasses and seaweeds [Internet]. Vol. 53, botm. 2010. p. 251–7. Available from: http://dx.doi.org/10.1515/bot.2010.032
Stabili L, Acquaviva MI, Angilè F, Cavallo RA, Cecere E, Del Coco L, et al. Screening of Chaetomorpha linum Lipidic Extract as a New Potential Source of Bioactive Compounds [Internet]. Vol. 17, Marine Drugs. 2019. p. 313. Available from: http://dx.doi.org/10.3390/md17060313
Senthilkumar P, Sudha S. Antioxidant and Antibacterial Properties of Methanolic Extract of Green Seaweed Chaetomorpha linum From Gulf of Mannar: Southeast Coast of India [Internet]. Vol. 5, Jundishapur Journal of Microbiology. 2012. p. 411–5. Available from: http://dx.doi.org/10.5812/jjm.3400
Haq SH, Al-Ruwaished G, Al-Mutlaq MA, Naji SA, Al-Mogren M, Al-Rashed S, et al. Antioxidant, Anticancer Activity and Phytochemical Analysis of Green Algae, Chaetomorpha Collected from the Arabian Gulf [Internet]. Vol. 9, Scientific Reports. 2019. Available from: http://dx.doi.org/10.1038/s41598-019-55309-1
Farasat M, Khavari-Nejad RA, Nabavi SMB, Namjooyan F. Antioxidant properties of some filamentous green algae (Chaetomorpha Genus) [Internet]. Vol. 56, Brazilian Archives of Biology and Technology. 2013. p. 921–7. Available from: http://dx.doi.org/10.1590/s1516-89132013000600005
Vadivel JK, Govindarajan M, Somasundaram E, Muthukrishnan A. Mast cell expression in oral lichen planus: A systematic review. J Investig Clin Dent. 2019 Nov;10(4):e12457.
Panda S, Jayakumar ND, Sankari M, Varghese SS, Kumar DS. Platelet rich fibrin and xenograft in treatment of intrabony defect. Contemp Clin Dent. 2014 Oct;5(4):550–4.
Mehta M, Dhanjal DS, Paudel KR, Singh B, Gupta G, Rajeshkumar S, et al. Cellular signalling pathways mediating the pathogenesis of chronic inflammatory respiratory diseases: an update. Inflammopharmacology. 2020 Aug;28(4):795–817.
Venkatesan J, Singh SK, Anil S, Kim SK, Shim MS. Preparation, Characterization and Biological Applications of Biosynthesized Silver Nanoparticles with Chitosan-Fucoidan Coating. Molecules [Internet]. 2018 Jun 12;23(6). Available from: http://dx.doi.org/10.3390/molecules23061429
Bhansali KJ, Balinge KR, Raut SU, Deshmukh SA, Senthil Kumar M, Ramesh Kumar C, et al. Visible light assisted sulfonic acid-functionalized porphyrin comprising benzimidazolium moiety for photocatalytic transesterification of castor oil. Fuel. 2021 Nov 15;304:121490.
Deepanraj B, Senthilkumar N, Mala D, Sathiamourthy A. Cashew nut shell liquid as alternate fuel for CI engine—optimization approach for performance improvement. Biomass Conversion and Biorefinery. 2022 May 1;12(5):1715–28.
Kamath M, Rao SK, Jaison, Sridhar, Kasthuri, Gopinath, et al. Melatonin delivery from PCL scaffold enhances glycosaminoglycans deposition in human chondrocytes – Bioactive scaffold model for cartilage regeneration. Process Biochem. 2020 Dec;99:36–47.
Paramasivam A, Priyadharsini JV. Novel insights into m6A modification in circular RNA and implications for immunity [Internet]. Vol. 17, Cellular & Molecular Immunology. 2020. p. 668–9. Available from: http://dx.doi.org/10.1038/s41423-020-0387-x
Li Z, Veeraraghavan VP, Mohan SK, Bolla SR, Lakshmanan H, Kumaran S, et al. Apoptotic induction and anti-metastatic activity of eugenol encapsulated chitosan nanopolymer on rat glioma C6 cells via alleviating the MMP signaling pathway [Internet]. Vol. 203, Journal of Photochemistry and Photobiology B: Biology. 2020. p. 111773. Available from: http://dx.doi.org/10.1016/j.jphotobiol.2019.111773
Nambi G, Kamal W, Es S, Joshi S, Trivedi P. Spinal manipulation plus laser therapy versus laser therapy alone in the treatment of chronic non-specific low back pain: a randomized controlled study. Eur J Phys Rehabil Med. 2018 Dec;54(6):880–9.