An In Vitro Investigation of the Effects of Periostin on Osteoblastic Adhesion and Proliferation on Collagen Matrices

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Pragyan Swain
Ranishree Behera
Saswati Mohanty
Shanvi
Anindita Ghosh
Bhumika J. Patel
Kapil Paiwal

Abstract

Purpose:The goal of the research was to determine how osteoblast cell adhesion, proliferation, and activity were affected when periostin was impregnated into various collagen matrices.


Materials and Methods:Onto two distinct collagen matrices, These are the steps used to cultivate and seed Saos-2 osteoblast cells: “Absorbable collagen sponges (ACS) are in Group A, absorbable collagen sponges impregnated with human periostin recombinant are in Group B, nanocrystalline hydroxyapatite collagen (NcHC) is in Group C, and NcHC impregnated with human periostin recombinant is in Group D. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test was performed on days 2, 5, and 7 to evaluate cell viability, adhesion, and proliferation.” Alkaline phosphatase (ALP) test was used to examine osteoblast activity in the research groups.


Results:On days 2, 5, and 7 the osteoblast adhesion in the periostin-treated absorbable collagen matrices was statistically significantly higher than that in the untreated NcHC (P=0.001). According to the results of the ALP test, the osteoblast activity in the periostin-treated ACS was higher than that in the periostin-treated NcHC.


Conclusion:


It is clear from the results of this research that Periostin plays a substantial part in regulating the cellular response of the osteoblast cells. Moreover, it has been shown that adding periostin to the ACS improves the osteoblast-like Saos-2 cells' cell survival, proliferation, and adhesion.

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How to Cite
Swain, P. ., Behera, R. ., Mohanty, S. ., Shanvi, Ghosh , A. ., J. Patel, B. ., & Paiwal , K. . (2023). An In Vitro Investigation of the Effects of Periostin on Osteoblastic Adhesion and Proliferation on Collagen Matrices. Journal of Coastal Life Medicine, 11(1), 1345–1352. Retrieved from https://www.jclmm.com/index.php/journal/article/view/519
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