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Background: In the last few years, various research has focused on the potential of herbal medicines as an alternative therapy for cancer. This is due in large part to the fact that these medications have very few side effects on healthy cells. Cherimoya, or Annona cherimola mill, is a natural fruit that is edible and recognized to exhibit several biological functions due to its significant content of phytochemical components. The anti-cancer effects of A. cherimola ethanolic leaf extract on leukemia have been described in previous investigations. The purpose of this in-vitro research is to investigate the possible anti-cancer efficacy of several solvent extracts of Annona cherimola mill in breast cancer cell lines, namely MCF-7. Methods: Cell viability assays were used to assess the antiproliferative effects of different solvent extracts. The purpose of this study was to analyze the aqueous extract of A.cherimola mill for cell viability, cell cycle, apoptosis detection, intracellular reactive oxygen species measurement, and protein characterization using western blotting. Results: Cell viability was reduced by 47% treated with aqueous extract, demonstrating its selectivity for the line of breast cancer cells. MCF-7. The IC25 for the extract was 0.43 mg/ml (MCF-7). Conclusion: Our findings reveal that the aqueous extract of A. cherimola mill has anti-tumor properties by selectively inhibiting the breast cancer cells (MCF-7) and promoting their apoptosis.
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