Diplocyclos Palmitus- A Pharmacognostical, in Silico and Invitro Antifungal Evaluation

Abstract

Aim: To carry out extraction, phytochemical evaluation, and in vitro & in silico anti-fungal evaluation of the leaves of Diplocyclos palmatus.

Objective: This study is undertaken to carry out extraction of the leaves of Diplocyclos palmatus and to evaluate its antifungal efficacy by In-silico and In-vivo studies.

Methodology: The extraction of the leaves of Diplocyclos palmatus was carried out by the Cold maceration technique, using various solvents such as Chloroform, Methanol, Ethanol, and water. The phytochemical evaluation of each of these extracts was carried out and the extracts were compared for their practical yields. 

In-silico analysis was carried out using Pyrex software and the active constituents of Diplocyclos palmatus were docked to the binding site of the Orotidine 5'-phosphate decarboxylase (PDB ID:2CZD). The in-vitro antifungal study was performed by Well Diffusion Assay.

Results: The results depicted that the highest yield was obtained from the ethanolic extract of the leaves. The active constituent, Isoquercetin, depicted the best docking score of -8 Kcal/mol, followed by the standard drugs, Luliconazole and Terbinafine (-7.2 and -7.3 Kcal/mol, respectively). Subsequent In-vitro analysis established that substantial antifungal efficacy upon comparison to the standard was demonstrated by the ethanolic extract of Diplocyclos palmatus compared to chloroform extract (diameter of zone of inhibition: 20±0.43 mm).

Conclusion: The data indicated that the Substantial antifungal efficacy upon comparison to the standard (Luliconazole) and the marketed standard, (Terbinafine), was demonstrated by the ethanolic extracts It was also evident from the results that, as the concentration increased, the antifungal efficacy also increased.